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Botanical Research 2013
农杆菌介导DR1372基因转化甘蓝型油菜
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Abstract:
DR1372基因是在耐辐射奇球菌(Deinococcus radiodurans, DR)基因组中克隆得到的一个基因，其蛋白序列存在一个WHy功能域，此功能域可能参与油菜的抗旱过程。本研究首先构建植物DR1372- pBI121表达载体，然后利用农杆菌介导法将目的基因DR1372成功转入甘蓝型油菜中，并对影响油菜再生频率以及遗传转化效率的几个因素进行了探讨，初步建立了油菜转DR1372基因遗传转化体系，为DR1372基因的功能研究奠定了基础。
The DR1372 containing a special domain called WHy was cloned from Deinococcus radiodurans. This WHy domain may be involved in the mechanism of drought resistance. In this study, the plant expression vector DR1372-pBI121 was constructed and introduced into Brassica napus L. mediated by Agrobacteria. Several factors affecting genetic transformation efficiency were explored and the Agrobacterium-mediated genetic transformation system was set up in Brassica napus. This study provides essential materials for later research on DR1372 gene.
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