%0 Journal Article
%T 农杆菌介导DR1372基因转化甘蓝型油菜<br>
Preliminary Study on Agrobacterium-Mediated 
Transformation of DR1372 Gene into Brassica napus L.
%A 张思维
%A 周文波
%A 张新
%A 陈翠娜
%A 代其林
%A 王劲
%J Botanical Research
%P 40-45
%@ 2168-5673
%D 2013
%I Hans Publishing
%R 10.12677/BR.2013.21007
%X <p style=\"text-align:justify;\">
	DR1372基因是在耐辐射奇球菌(Deinococcus radiodurans, DR)基因组中克隆得到的一个基因，其蛋白序列存在一个WHy功能域，此功能域可能参与油菜的抗旱过程。本研究首先构建植物DR1372- pBI121表达载体，然后利用农杆菌介导法将目的基因DR1372成功转入甘蓝型油菜中，并对影响油菜再生频率以及遗传转化效率的几个因素进行了探讨，初步建立了油菜转DR1372基因遗传转化体系，为DR1372基因的功能研究奠定了基础。&lt;br/&gt;The DR1372 containing a special domain called WHy was cloned from Deinococcus radiodurans. This WHy domain may be involved in the mechanism of drought resistance. In this study, the plant expression vector DR1372-pBI121 was constructed and introduced into Brassica napus L. mediated by Agrobacteria. Several factors affecting genetic transformation efficiency were explored and the Agrobacterium-mediated genetic transformation system was set up in Brassica napus. This study provides essential materials for later research on DR1372 gene.
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%K DR1372；油菜；农杆菌介导；遗传转化<br>
DR1372
%K Brassica napus L.
%K Genetic Transformation
%K Drought-Tolerance
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=9325