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优良品种龙滩珍珠李自花结实分子机理初探
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Abstract:
本研究主要以广西本地生长的优质的具自花结实李品种龙滩珍珠李为材料，根据李属雌蕊S-RNase基因的序列信息设计了一对特异性保守引物，对龙滩珍珠李基因组DNA进行PCR扩增，并对扩增产物进行克隆、测序以及序列分析，成功克隆出两个S-RNase基因片段，其中一个为在中国李中首次发现并命名为PsS-RNase-28，大小为727 bp，其中包含有367 bp的内含子，仅有360 bp的外显子编码序列，与黑刺李(Prunus spinosa)S-RNase S1基因(EF636467.1)同源率达到98%，与其它李S- RNase基因的同源率都达到79%以上。另一个扩增片段大小为718 bp，包含有343 bp的内含子，其中仅有375 bp的外显子编码序列，与中国李(Prunus salicina)PsS-RNase-h基因(AB084148.1)相似性达到99%，与其它李
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