%0 Journal Article
%T 优良品种龙滩珍珠李自花结实分子机理初探<br>Pre-Study on the Molecular Mechanism of Self-Compatibility in Longtanzhenzhuli
%A 叶燕萍
%A 黄绍西
%A 孟丽萍
%J Hans Journal of Agricultural Sciences
%P 49-57
%@ 2164-5523
%D 2012
%I Hans Publishing
%R 10.12677/HJAS.2012.23009
%X <span style=\"font-family:宋体;font-size:10pt;\">本研究主要以广西本地生长的优质的具自花结实李品种龙滩珍珠李为材料，根据李属雌蕊</span><i><span style=\"font-size:10pt;\">S-RNase</span></i><span style=\"font-family:宋体;font-size:10pt;\">基因的序列信息设计了一对特异性保守引物，对龙滩珍珠李基因组</span><span style=\"font-size:10pt;\">DNA</span><span style=\"font-family:宋体;font-size:10pt;\">进行</span><span style=\"font-size:10pt;\">PCR</span><span style=\"font-family:宋体;font-size:10pt;\">扩增，并对扩增产物进行克隆、测序以及序列分析，成功克隆出两个</span><i><span style=\"font-size:10pt;\">S-RNase</span></i><span style=\"font-family:宋体;font-size:10pt;\">基因片段，其中一个为在中国李中首次发现并命名为</span><i><span style=\"font-size:10pt;\">PsS-RNase-</span></i><span style=\"font-size:10pt;\">28</span><span style=\"font-family:宋体;font-size:10pt;\">，大小为</span><span style=\"font-size:10pt;\">727 bp</span><span style=\"font-family:宋体;font-size:10pt;\">，其中包含有</span><span style=\"font-size:10pt;\">367 bp</span><span style=\"font-family:宋体;font-size:10pt;\">的内含子，仅有</span><span style=\"font-size:10pt;\">360 bp</span><span style=\"font-family:宋体;font-size:10pt;\">的外显子编码序列，与黑刺李</span><span style=\"font-size:10pt;\">(</span><i><span style=\"font-size:10pt;\">Prunus spinosa</span></i><span style=\"font-size:10pt;\">)</span><i><span style=\"font-size:10pt;\">S-RNase S1</span></i><span style=\"font-family:宋体;font-size:10pt;\">基因</span><span style=\"font-size:10pt;\">(</span><span style=\"font-size:10pt;\">EF636467.1</span><span style=\"font-size:10pt;\">)</span><span style=\"font-family:宋体;font-size:10pt;\">同源率达到</span><span style=\"font-size:10pt;\">98%</span><span style=\"font-family:宋体;font-size:10pt;\">，与其它李</span><i><span style=\"font-size:10pt;\">S- RNase</span></i><span style=\"font-family:宋体;font-size:10pt;\">基因的同源率都达到</span><span style=\"font-size:10pt;\">79%</span><span style=\"font-family:宋体;font-size:10pt;\">以上。另一个扩增片段大小为</span><span style=\"font-size:10pt;\">718 bp</span><span style=\"font-family:宋体;font-size:10pt;\">，包含有</span><span style=\"font-size:10pt;\">343 bp</span><span style=\"font-family:宋体;font-size:10pt;\">的内含子，其中仅有</span><span style=\"font-size:10pt;\">375 bp</span><span style=\"font-family:宋体;font-size:10pt;\">的外显子编码序列，与中国李</span><span style=\"font-size:10pt;\">(<i>Prunus salicina</i>)<i>PsS-RNase</i>-<i>h</i></span><span style=\"font-family:宋体;font-size:10pt;\">基因</span><span style=\"font-size:10pt;\">(</span><span style=\"font-size:10pt;\">AB084148.1</span><span style=\"font-size:10pt;\">)</span><span style=\"font-family:宋体;font-size:10pt;\">相似性达到</span><span style=\"font-size:10pt;\">99%</span><span style=\"font-family:宋体;font-size:10pt;\">，与其它李</span><i><span
%K 龙滩珍珠李；自交不亲和；自花结实；S-RNase(S)基因<br>Longtanzhenzhuli
%K Self-Incompatibility
%K Self-Compatibility
%K S-Rnase Genes
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=2936