Detection of Full Length Hb S Gene (1.2 Kb) in One- and Two-cell Embryo Originated from Hamster Oocyte and Human Spermatozoa by Using Nested-PCR

The polymerase chain reaction (PCR) is a versatile method for rapid amplification of selected DNA segments. The aim of this study was to amplify the full length of Hepatitis B Surface (HBS) gene in one- and two-cell embryo (single embryo) after introducing motile human sperm carrying hepatitis B virus (HBV) DNA into zona-free hamster oocytes via in vitro fertilization (IVF) technique. Human sperm-mediated HBV genome was delivered into zona-free hamster oocytes by IVF. The results revealed that full length HBS could be amplified in one-and two-cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences.