%0 Journal Article
%T Detection of Full Length Hb S Gene (1.2 Kb) in One- and Two-cell Embryo Originated from Hamster Oocyte and Human Spermatozoa by Using Nested-PCR
%A Bahy A. Ali
%A Halima H. Salem
%A Tian H. Huang
%A Qing D. Xie
%J Journal of Medical Sciences
%D 2006
%I 
%X The polymerase chain reaction (PCR) is a versatile method for rapid amplification of selected DNA segments. The aim of this study was to amplify the full length of Hepatitis B Surface (HBS) gene in one- and two-cell embryo (single embryo) after introducing motile human sperm carrying hepatitis B virus (HBV) DNA into zona-free hamster oocytes via in vitro fertilization (IVF) technique. Human sperm-mediated HBV genome was delivered into zona-free hamster oocytes by IVF. The results revealed that full length HBS could be amplified in one-and two-cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences.
%K In vitro fertilization
%K HBS gene
%K full length
%K hamster ova
%K human spermatozoa
%K nested PCR
%U http://docsdrive.com/pdfs/ansinet/jms/2006/1015-1020.pdf