Determination of PGlu Content in Solid Substance of Tanreqing Injection by RPHPLC

The content of PGlu in solid substance in Tanreqing injection dose was analyzed. The content of PGlu in solid substance in Tanreqing by RPHPLC was determined, and defined the content of PGlu in the solid substance of different batches Tanreqing by comparing with reference of PGlu. In this study the Chromatographic conditions was Sionchrom ODSBP(200 mm×4.6 mm,5 μm)column, acetonitrile-3.5 mmol/L phosphate buffer(volume ratio 1:99) with pH 3 as mobile phase, the flow rate was 1.0 mL/min, detection wavelength was set at 205 nm, the temperature of column was set at 27 ℃. The results showed that the Content of PGlu in different batches were determined in the range of 0.5 %～1.2 %, the average value was 0.86%, it was indicated that the content of PGlu in Tanreqing injection dose differs slightly in different batches. The method established in the paper was effective and stable, which is quite important to the quality control of Tanyeqing injection dose.