Simultaneous determination of anthocyanoside and beta-carotene by third-derivative ultraviolet spectrophotometry

Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a third-derivative spectrophotometry method was used for simultaneous determination of anthocyanoside and beta-carotene using the zero-crossing technique. The measurements were carried out at wavelengths of 625 and 540 nm for anthocyanoside and beta-carotene respectively. The method was found to be linear (r2>0.999) in the range of 125-750 μg/mL for anthocyanoside in the presence of 25 μg/mL beta-carotene at 625 nm. The same linear correlation was also obtained (r2>0.997) in the range of 6.25-37.50 μg/mL for beta-carotene in the presence of 500 μg/mL of anthocyanoside at 540 nm. The limit of determination was 125 and 6.25 μg/mL for anthocyanoside and beta-carotene respectively. The method was successfully applied for simultaneous determination of anthocyanoside and beta-carotene in pharmaceutical preparations without any interferences from excipients.