%0 Journal Article
%T Simultaneous determination of anthocyanoside and beta-carotene by third-derivative ultraviolet spectrophotometry
%A "Effat Souri
%A Hassan Jalalizadeh
%A Hassan Farsam
%A Hossein Rezwani
%J DARU : Journal of Pharmaceutical Sciences
%D 2005
%I BioMed Central
%X Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a third-derivative spectrophotometry method was used for simultaneous determination of anthocyanoside and beta-carotene using the zero-crossing technique. The measurements were carried out at wavelengths of 625 and 540 nm for anthocyanoside and beta-carotene respectively. The method was found to be linear (r2>0.999) in the range of 125-750 ¦Ìg/mL for anthocyanoside in the presence of 25 ¦Ìg/mL beta-carotene at 625 nm. The same linear correlation was also obtained (r2>0.997) in the range of 6.25-37.50 ¦Ìg/mL for beta-carotene in the presence of 500 ¦Ìg/mL of anthocyanoside at 540 nm. The limit of determination was 125 and 6.25 ¦Ìg/mL for anthocyanoside and beta-carotene respectively. The method was successfully applied for simultaneous determination of anthocyanoside and beta-carotene in pharmaceutical preparations without any interferences from excipients.
%K Anthocyanoside
%K Beta-carotene
%K Third derivative spectrophotometry
%K Simultaneous
%U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/494.pdf&manuscript_id=494