A NOVEL INTEGRON IN THE GENOME OF ESCHERICHIA COL I ISOLATED FROM INDONESIAN MONITOR LIZARD (VARANUS SPP).

The ge no ty pe o f an ti bi ot ic re si sta nce in nat ural i solat es of Es ch er ichi a c oli was d ete rmin ed through integron detection and characterization of the associat ed antibiotic re sistance. E. coli SG2 is olated f rom Vara nus salvator of Java demonstrated re si stance to specti no myci n (5 0ng/ml) and st re pt omycin (SOng/ml). Inte gr on detection indi ca te d th at eight isol at es out of nine E. c oli isola tes possessed a conserved segment of the integron. Amplification of the inserted cassette of the integro n in this SG2 isolate y ielded a 1-kb DNA fragment. S equence analy ses indica ted t hat this fragment was homologous with aad ge ne, which confirm ed the re sistance to s pec tinomycin /stre ptom ycin. This i s t he first report on the presence of inte gron in t he E. coli isolated from the envi ronment.