%0 Journal Article
%T A NOVEL INTEGRON IN THE GENOME OF ESCHERICHIA COL I ISOLATED FROM INDONESIAN MONITOR LIZARD (VARANUS SPP).
%A STI PUSPIT ASAR
%A WALTER ERDEL EN
%A A NTONI US SUWANT O
%A AMAR ILA MA LIK
%J BIOTROPIA : the Southeast Asian Journal of Tropical Biology
%D 2001
%I SEAMEO, BIOTROP
%X The ge no ty pe o f an ti bi ot ic re si sta nce in nat ural i solat es of Es ch er ichi a c oli was d ete rmin ed through integron detection and characterization of the associat ed antibiotic re sistance. E. coli SG2 is olated f rom Vara nus salvator of Java demonstrated re si stance to specti no myci n (5 0ng/ml) and st re pt omycin (SOng/ml). Inte gr on detection indi ca te d th at eight isol at es out of nine E. c oli isola tes possessed a conserved segment of the integron. Amplification of the inserted cassette of the integro n in this SG2 isolate y ielded a 1-kb DNA fragment. S equence analy ses indica ted t hat this fragment was homologous with aad ge ne, which confirm ed the re sistance to s pec tinomycin /stre ptom ycin. This i s t he first report on the presence of inte gron in t he E. coli isolated from the envi ronment.
%K Integron / antibiotic re si stance / Es cher ic hia coli
%U http://journal.biotrop.org/index.php/biotropia/article/view/163/133