Bioinformatics Analysis of Tryptophan Synthase beta Chain in an Archaea

Structure of Tryptophan Synthase was reported from Salmonella typhimurium having α2β2 complex structure,Crystal structure of the Tryptophan Synthase β2 subunit reported from Pyrococcus furiosus . It has two beta subunits coding for two different genes, beta 1 and beta 2 subunits are coded by PF1592 and PF1706. This is probably due to gene duplication .The length of beta 1 and 2 subunit is 446, 388 amino acids. Present study deals with the tryptophan synthase beta chain amino acid sequence from 13 Archaea available in KEGG. Pyrococcus furiosus taken as reference organism for comparative genomics of Tryptophan synthase, available data used for comparative genomics was interrogated using bioinformatic tools. Amino acid sequence of tryptophan synthase beta 2 subunit of Pyrococcus furiosus from the KEGG was taken as the standard sequence followed by Pair-wise alignments,multiple sequence alignment using BLAST 2, Clustal X and phylogenitic analysis made by tree view.