Screening for Bioactive Compound Group in Parmotrema tinctorum’s Extract and Bioactivity Test of Dichloromethane Extract as Anti-tuberculosis Against Mycobacterium tuberculosis H37RV and Toxicity Against Artemia salina.

The aims of this research were to estimate compound group in P. tinctorum’s extract, testtheir dichloromethane extract bioactivity as anti-tuberculosis against Mycobacteriumtuberculosis H37RV and their toxicity against Artemia salina. The result of specific colortest indicated that phenolic, depside and many β-orcinol depsidone compound were presentin hexane, dichloromethane, ethyl acetate and acetone extracts whereas depsidone anddepside with two free hydroxyl groups on meta position were present only in ethyl acetateand acetone extracts. The result of bioactivity test showed that dichloromethane extract wastoxic to Artemia salina, (LC50 = 57.016 μg/ml) but it could not inhibit Mycobacteriumtuberculosis H37RV growth.