Ophiostoma ips asociado al insecto descortezador (Dendroctonus adjunctus) del pino de las alturas (Pinus hartwegii) Ophiostoma ips associated with the bark beetle (Dendroctonus adjunctus) in hartweg pine

En el "pino de las alturas" (Pinus hartwegii Lindl) se ha reportado la presencia del descortezador Dendroctonus adjunctus asociado al género Ophiostoma spp., causante del manchado azul de la madera. El objetivo del presente estudio fue identificar y caracterizar la especie de Ophiostoma asociada al descortezador D. adjunctus en P hartwegii. Se colectaron galerías e insectos en la Estación Forestal Experimental Zoquiapan (EFEZ) de la Universidad Autónoma Chapingo, en Zoquiapan, Puebla. Se aisló e identificó morfológicamente a Graphilbum sp. en galerías e insectos y Ophiostoma ips solamente en galerías. Además, se caracterizaron molecularmente mediante la técnica de reacción en cadena de la polimerasa (PCR). El análisis molecular corroboró la identificación morfológica. Se concluye que el hongo corresponde a Ophiostoma ips (teleomorfo) y Graphilbum sp. su anamorfo. Este es el primer reporte en México de O. ips asociado a P hartwegii. In the "pino de las alturas" (Pinus hartwegii Lindl) it has been reported the presence of the bark beetle Dendroctonus adjunctus associated with the genus Ophiostoma spp., which causes the blue-stain of the wood. The objective of this study was to identify and characterize the Ophiostoma species associated with the bark beetle D. adjunctus in P. hartwegii. Galleries and insects were collected in the Zoquiapan Experimental Forest Station (ZEFS), of the Universidad Autonoma Chapingo, in Zoquiapan, Puebla. It was isolated from galleries and insects the morphologically identified fungi Graphilbum sp. and Ophiostoma ips, the latter from galleries only. In addition, molecular characterization was developed by the polymerase chain reaction technique (PCR). Molecular analysis confirmed the morphological identification. It was conclude that this fungus corresponds to Ophiostoma ips which is found on both stages, the teleomorph and its anamorph (Graphilbum sp.). This is the first report of O. ips in Mexico associated with P. hartwegii.