%0 Journal Article
%T Ophiostoma ips asociado al insecto descortezador (Dendroctonus adjunctus) del pino de las alturas (Pinus hartwegii) Ophiostoma ips associated with the bark beetle (Dendroctonus adjunctus) in hartweg pine
%A Omar Alejandro P谷rez-Vera
%A Dionicio Alvarado-Rosales
%A Elizabeth C芍rdenas-Soriano
%A Armando Equihua-Mart赤nez
%J Revista mexicana de micolog赤a
%D 2009
%I Sociedad Mexicana de Micolog赤a
%X En el "pino de las alturas" (Pinus hartwegii Lindl) se ha reportado la presencia del descortezador Dendroctonus adjunctus asociado al g谷nero Ophiostoma spp., causante del manchado azul de la madera. El objetivo del presente estudio fue identificar y caracterizar la especie de Ophiostoma asociada al descortezador D. adjunctus en P hartwegii. Se colectaron galer赤as e insectos en la Estaci車n Forestal Experimental Zoquiapan (EFEZ) de la Universidad Aut車noma Chapingo, en Zoquiapan, Puebla. Se aisl車 e identific車 morfol車gicamente a Graphilbum sp. en galer赤as e insectos y Ophiostoma ips solamente en galer赤as. Adem芍s, se caracterizaron molecularmente mediante la t谷cnica de reacci車n en cadena de la polimerasa (PCR). El an芍lisis molecular corrobor車 la identificaci車n morfol車gica. Se concluye que el hongo corresponde a Ophiostoma ips (teleomorfo) y Graphilbum sp. su anamorfo. Este es el primer reporte en M谷xico de O. ips asociado a P hartwegii. In the "pino de las alturas" (Pinus hartwegii Lindl) it has been reported the presence of the bark beetle Dendroctonus adjunctus associated with the genus Ophiostoma spp., which causes the blue-stain of the wood. The objective of this study was to identify and characterize the Ophiostoma species associated with the bark beetle D. adjunctus in P. hartwegii. Galleries and insects were collected in the Zoquiapan Experimental Forest Station (ZEFS), of the Universidad Autonoma Chapingo, in Zoquiapan, Puebla. It was isolated from galleries and insects the morphologically identified fungi Graphilbum sp. and Ophiostoma ips, the latter from galleries only. In addition, molecular characterization was developed by the polymerase chain reaction technique (PCR). Molecular analysis confirmed the morphological identification. It was conclude that this fungus corresponds to Ophiostoma ips which is found on both stages, the teleomorph and its anamorph (Graphilbum sp.). This is the first report of O. ips in Mexico associated with P. hartwegii.
%K Graphilbum
%K hongo
%K insecto
%K PCR
%K Graphilbum
%K fungi
%K insect
%K PCR
%U http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0187-31802009000200002