Snail is one of the important components of an aquatic ecosystem, it acts as intermediate host of Fasciola species. Control of snail population below a certain threshold level is one of the important methods in the campaign to reduce the incidence of fascioliasis. Life cycle of the parasite can be interrupted by killing the snail or Fasciola larva redia and cercaria in the snail body. In vivo and in vitro toxicity of the plant products and their active component such as citral, ferulic acid, umbelliferone, azadirachtin, and allicin against larva of Fasciola in infected snail Lymnaea acuminata were tested. Mortality of larvae were observed at 2？h, 4？h, 6？h, and 8？h, of treatment. In in vivo treatment, azadirachtin caused highest mortality in redia and cercaria larva (8？h, LC50 0.11, and 0.05？mg/L) whereas in in vitro condition allicin was highly toxic against redia and cercaria (8？h, LC50 0.01, and 0.009？mg/L). Toxicity of citral was lowest against redia and cercaria larva. 1. Introduction Fascioliasis is a worldwide zoonotic disease caused by Fasciola hepatica and Fasciola gigantica (family fasciolidae) [1]. F. hepatica has worldwide distribution but predominates in temperate zones while F. gigantica is found primarily in tropical regions [2–4]. The definite host is very broad and includes many herbivorous mammals including humans. Human fascioliasis has been reported in 51 different countries from five continents [4]. Fascioliasis is now recognized as an emerging human disease. World health organization has estimated that 2.4 million people are infected with Fasciola and a further 180 million are at risk of infection [1]. Singh and Agarwal [5] reported that 94% of buffaloes slaughtered in local slaughtered house in Gorakhpur district are infected with F. gigantica. In northern India Lymnaea acuminata is the intermediate hosts of the Fasciola species [2]. Although control of snail population below a threshold level is one of the important methods for effective control of fascioliasis [6–9], yet snails are one of the important components in the aquatic ecosystem. Release of molluscicides in aquatic system for snail control also affects the other nontarget organism. The Fasciola larval stage sporocyst, redia, and cercaria in the snail body are in division phase of F. gigantica. If these larvae will be destroyed by plant molluscicides at sublethal concentration in the snail body, the rate of infection can be reduced without killing the snail. Different plants-derived molluscicides and their active component such as citral, ferulic acid, umbelliferone,
References
[1]
World Health Organization, Report of the WHO Informal Meeting on Use of Triclabendazole in Fascioliasis Control, WHO, Geneva, Switzerland, 2006.
[2]
R. A. Agarwal and D. K. Singh, “Harmful gastropods and their control,” Acta Hydrochimica et Hydrobiologica, vol. 16, pp. 113–138, 1988.
[3]
S. Mas-Coma, M. D. Bargues, and M. A. Valero, “Fascioliasis and other plant-borne trematode zoonoses,” International Journal for Parasitology, vol. 35, no. 11-12, pp. 1255–1278, 2005.
[4]
M. S. Mas-Coma, J. G. Esteban, and M. D. Bargues, “Epidemiology of human fascioliasis: a review and proposed new classification,” Bulletin of the World Health Organization, vol. 77, no. 4, pp. 340–346, 1999.
[5]
O. Singh and R. A. Agarwal, “Toxicity of certain pesticides to two economic species of snail in northern India,” Journal of Economic Entomology, vol. 74, pp. 568–571, 1981.
[6]
A. Singh, D. K. Singh, T. N. Misra, and R. A. Agarwal, “Molluscicides of plant origin,” Biological Agriculture and Horticulture, vol. 13, no. 3, pp. 205–252, 1996.
[7]
P. Kumar and D. K. Singh, “Molluscicidal activity of Ferula asafoetida, Syzygium aromaticum and Carum carvi and their active components against the snail Lymnaea acuminata,” Chemosphere, vol. 63, no. 9, pp. 1568–1574, 2006.
[8]
P. Kumar, V. K. Singh, and D. K. Singh, “Kinetics of enzyme inhibition by active molluscicidal agents ferulic acid, umbelliferone, eugenol and limonene in the nervous tissue of snail Lymnaea acuminata,” Phytotherapy Research, vol. 23, no. 2, pp. 172–177, 2009.
[9]
P. Jaiswal and D. K. Singh, “Molluscicidal activity of nutmeg and mace (Myristica fragrans houtt.) against the vector snail Lymnaea acuminata,” Journal of Herbs, Spices and Medicinal Plants, vol. 15, no. 2, pp. 177–186, 2009.
[10]
K. Singh, A. Singh, and D. K. Singh, “Molluscicidal activity of different combinations of the plant products used in the molluscicide Pestoban,” Biological Agriculture and Horticulture, vol. 12, no. 3, pp. 253–261, 1995.
[11]
V. K. Singh and D. K. Singh, “Characterization of allicin as a molluscicidal agent in Allium sativum (Garlic),” Biological Agriculture and Horticulture, vol. 12, no. 2, pp. 119–131, 1995.
[12]
S. Singh, V. K. Singh, and D. K. Singh, “Molluscicidal activity of some common spice plants,” Biological Agriculture and Horticulture, vol. 14, no. 3, pp. 237–249, 1997.
[13]
J. L. Robertson, R. M. Russell, H. K. Preciter, and N. E. Savin, Bioassay with Arthropods Data, Taylar and Francis; CRC Press, Boca Raton, Fla, USA, 2nd edition, 2007.
[14]
R. R. Sokal and F. J. Rohlf, Introduction of Biostatistics, W.H. Freeman, San Francisco, Calif, USA,, 1996.
[15]
R. Kaleysa Raj, “Screening of indigenous plants for anthelmintic action against human Ascaris lumbricoides: part II,” Indian Journal of Physiology and Pharmacology, vol. 19, no. 1, pp. 47–50, 1975.
[16]
K. M. Nadkarni, Indian Materia Medica. Vol I and II Popular Prakashan, Private Limited Bombay, Mumbai, India, 1976.
[17]
P. Schavenberg and F. Paris, Guide to Medicinal Plants, Lutterworth Press, London, UK, 1977.
[18]
M. J. V. Steenis-Kruseman, “Select Indonesian medicinal plants organize,” Science Research Indonesia Bulltin, vol. 18, p. 31, 1953.
[19]
H. A. Hoppe, Drogenkunde, Vol. I .Angiosperms, Walter De Gruyter, Berlin, Germany, 8th edition, 1975.
[20]
K. R. Kirtikar and B. D. Basu, Indian Medicinal Plants. Part II, Indian Press, 1981.
[21]
R. Kaleysa Raj, “Screening of some indigenous plants for anthelmintic action against human Ascaris lumbricoides,” Indian Journal of Physiology and Pharmacology, vol. 18, no. 2, pp. 129–131, 1974.
[22]
C. Goto, S. Kasuya, K. Koga, H. Ohtoma, and N. Kagei, “Lethal efficacy of extract from Zingiber officinale (traditional Chinese medicine) or [6]-shogaol and [6]-gingerol in Anisakis larvae in vitro,” Parasitology Research, vol. 76, no. 8, pp. 653–656, 1990.
[23]
A. Datta and N. C. Sukul, “Antifilarial effect of Zingiber officinale on Dirofilaria immitis,” Journal of Helminthology, vol. 61, no. 3, pp. 268–270, 1987.
[24]
C. O. Adewunmi, B. O. Oguntimein, and P. Furu, “Molluscicidal and antischistosomal activities of Zingiber officinale,” Planta Medica, vol. 56, no. 4, pp. 374–376, 1990.
[25]
D. Eigner and D. Scholz, “Ferula asa-foetida and Curcuma longa in traditional medical treatment and diet in Nepal,” Journal of Ethnopharmacology, vol. 67, no. 1, pp. 1–6, 1999.
[26]
M. Saleem, A. Alam, and S. Sultana, “Asafoetida inhibits early events of carcinogenesis: a chemopreventive study,” Life Sciences, vol. 68, no. 16, pp. 1913–1921, 2001.
[27]
H. Furukawa, S. Zenno, Y. Iwasawa, H. Morita, T. Yoshida, and T. Nagasawa, “Ferulic acid production from clove oil by pseudomonas fluorescens E118,” Journal of Bioscience and Bioengineering, vol. 96, no. 4, pp. 404–405, 2003.
[28]
M. Fatehi, F. Farifteh, and Z. F. Hassanabad, “Antispasmodic and hypotensive effects of Ferula Asafoetida gum extract,” Journal of Ethnopharmacology, vol. 91, no. 2-3, pp. 321–324, 2004.
[29]
L. M. Assis, C. M. L. Bevilequa, S. M. Morais, L. S. Vieira, C. T. C. Costa, and J. A. L. Souza, “Ovicidal and larvicidal activity in vitro of Spieled anthelia Linn. Extract on Haemonchus contorlus,” Veterinary Parasitology, vol. 117, pp. 43–49, 2003.
[30]
V. K. Singh, S. Singh, S. Singh, and D. K. Singh, “Effect of active molluscicidal component of spices on different enzyme activities and biogenic amine levels in the nervous tissue of Lymnaea acuminata,” Phytotherapy Research, vol. 13, no. 8, pp. 649–654, 1999.
[31]
M. Humiczewska, “Oxidative enzymes in the development of Fasciola hepatica L,” Folia Histochemica et Cytochemica, vol. 13, pp. 37–130, 1975.
[32]
A. G. M. Tielens, “Biochemistry of trematodes,” in Advances in Trematode Biology, B. Fried and T. K. Graczyk, Eds., pp. 309–343, CRC Press, Boca Raton, Fla, USA, 1997.
[33]
G. D. Schmidt and L. S. Roberts, Trematoda: From Function, and Classification of Digeneans Foundation of Parasitology, McGraw- Hill, New York, NY, USA, 6th edition, 2000.
