An Introduction to a Hyphenated Technique: HPLC-SPE-NMR

Liquid chromatography–solid phase extraction–nuclear magnetic resonance (HPLC-SPE-NMR), noveland highly promising hyphenated technique which is based on post-column analyte trapping by solidphaseextraction. The analytes are subsequently eluted from the SPE cartridges using deuteratedsolvents. This indirect HPLC-NMR hyphenation offers numerous advantages compared to direct HPLCNMRmethods. Multiple trapping leads to a dramatic increase of analyte amounts available for NMR,enabling acquisition of high-quality 2D NMR data within a short time. The well-defined NMR solventconditions make spectra comparisons feasible, which means databases and spectra catalogues.