This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin ( 1), alternariol ( 2), 3'-hydroxyalternariol monomethyl ether ( 3), and alternariol monomethyl ether ( 4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean ( Glycine max) cell cultures as a model. EC 50 values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds.
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