%0 Journal Article
%T Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
%A Gezimar D. de Souza
%A Axel Mith£¿fer
%A Cristina Daolio
%A Bernd Schneider
%A Edson Rodrigues-Filho
%J Molecules
%D 2013
%I MDPI AG
%R 10.3390/molecules18032528
%X This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin ( 1), alternariol ( 2), 3'-hydroxyalternariol monomethyl ether ( 3), and alternariol monomethyl ether ( 4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean ( Glycine max) cell cultures as a model. EC 50 values which range from 0.11 (¡À0.02) to 4.69 (¡À0.47) ¦ÌM showed the high cytotoxicity of these compounds.
%K LC-NMR
%K LC-SPE-NMR
%K mass spectrometry
%K alternariol
%K Alternaria
%K soybean
%K Glycine max
%K cell cultures
%K cytotoxicity
%U http://www.mdpi.com/1420-3049/18/3/2528