Estimation of Lomefloxacin hydrochloride in Bulk and Tablet dosage form by HPTLC method

Plan: Ananalytical method for the estimation of Lomefloxacin in bulk drug and pharmaceutical dosageforms by HPTLC is described.Prologue: Themethod is simple, fast and accurate and can be used for routineanalysis of commercial Lomefloxacin tablets.Methodology: Thedeveloped method used precoated silica gel 60F254 as stationary phase. Themobile phase used was a mixture of 2-Propanol: water: ammonia (86:8:6 %v/v/v).The RF value was 0.14.The detection of spots was carried out at 288nm. Thecalibration curve was found to be linear between 10 and 70 ng/spot with acorrelation coefficient of 0.9969. The limit of detection (LOD) and limit ofquantification (LOQ) were found to be 5 ng/spot and 10 ng/spot respectively.Outcome: Theproposed method has been applied successfully for the determination of Lomefloxacin inpharmaceutical dosage forms. No significant interference was observed fromexcipients, coloring and flavoring agents commonly used in the formulation. Themean recovery of drug from tablets was in the range of 100.8% to 102.7 %.Key Words:HPTLC,Lomefloxacin, Mobile phase, Stationary phase.