Research: SIMULTANEOUS ESTIMATION OF TURPENTINE OIL, CHLORBUTOL, PARA- DICHLOROBENZENE AND BENZOCAINE BY CAPILLARY GAS CHROMATOGRAPHY

This paper describes a new, simple, precise and accurate GC method for simultaneous estimation of Turpentine oil,Chlorbutol, Para- dichlorobenzene and Benzocaine in formulation with flame ionization detection (GC-FID). Abaseline separation of active drugs and internal standard was achieved in 12min. using BP1 (100% Dimethylpolysiloxane) capillary column. During analysis the oven temperature was programmed. Validation was carriedout in compliance with the International Conference on Harmonization guidelines. The proposed method wasvalidated for linearity, LOD, LOQ, accuracy, precision. It can be conveniently adopted for routine quality controlanalysis.