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Lymphoid Genes Expression in Mouse Embryonic Stem Cells with Differentiation on Feeder Free Condition

Keywords: Genes Expression , Mouse Embryonic Stem Cells , Differentiation , Feeder Free Condition

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Abstract:

Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR.

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