%0 Journal Article
%T Lymphoid Genes Expression in Mouse Embryonic Stem Cells with Differentiation on Feeder Free Condition
%A Majid Mossahebimohammadi
%A Saeid Kaviani
%A Mina Soufizomorrod
%A Masoud Soleimani
%J International Journal of Hematology-Oncology and Stem Cell Research
%D 2012
%I 
%X Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR.
%K Genes Expression
%K Mouse Embryonic Stem Cells
%K Differentiation
%K Feeder Free Condition
%U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/21109.pdf&manuscript_id=21109