Determination of Iohexol by High Performance Liquid Chromatography
高效液相色谱法测定非离子造影剂欧乃帕克

A fast, sensitive and reproducible method for the determination of iohexol by high performance liquid chromatography with narrow-bore column was developed. The method involved one step deproteinization of plasma by perchloric acid and a simple, isocratic elution system of 7% (v/v) methane solution. The linearity of the method ranged from 0.5 to 500 mg/L of iohexol with a correlation coefficient of more than 0.999 8. Minimum detection limit was 154 pg of the agent in plasma. Retention time for the contrast medium in the elution system was (3.46 +/- 0.02) min and a single run was less than 5 min. The relative standard deviation of iohexol in peak area was less than 4.3% for run to run within a day and 11.4% for day to day operations. The recoveries of iohexol in plasma were over 91.51% at low, middle and high concentrations.