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色谱 2003
Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography
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Abstract:
High performance liquid chromatography (HPLC) coupled wiht electro-spray ioniza tion mass spectrometry (ESI-MS) was used for the determination and identificat ion of theanine in tea without derivatization. A reversed-phase HPLC method was developed and the effects of the mobile phase composition on separation were investigated. Theanine can be separated completely from other components on a Sp herigel C 18 column with trifluoroacetic acid solution (pH 3 0) as mobile phase. The UV detection wavelength was set at 203 nm. The limit of detection wa s down to 1 75 ng. The method is simple and accurate.
