%0 Journal Article
%T Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography<br>高效液相色谱法测定茶叶中的茶氨酸
%A ZHU Xiaolan
%A CHEN Bo
%A LUO Xubiao
%A YAO Shouzhuo
%A <br>朱小兰
%A 陈波
%A 罗旭彪
%A 姚守拙
%J 色谱
%D 2003
%I 
%X High performance liquid chromatography (HPLC) coupled wiht electro-spray ioniza tion mass spectrometry (ESI-MS) was used for the determination and identificat ion of theanine in tea without derivatization. A reversed-phase HPLC method was developed and the effects of the mobile phase composition on separation were investigated. Theanine can be separated completely from other components on a Sp herigel C 18 column with trifluoroacetic acid solution (pH 3 0) as mobile phase. The UV detection wavelength was set at 203 nm. The limit of detection wa s down to 1 75 ng. The method is simple and accurate.
%K high performance liquid chromatography
%K liquid chromatography-mass spectrometry
%K theanine
%K tea<br>高效液相色谱法
%K 高效液相色谱质谱
%K 茶氨酸
%K 茶叶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8D8E5467D7572B8C&yid=D43C4A19B2EE3C0A&vid=659D3B06EBF534A7&iid=E158A972A605785F&sid=8ACD9060100C26F1&eid=EE23223CE4332BD7&journal_id=1000-8713&journal_name=色谱&referenced_num=10&reference_num=7