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色谱  2004 

Determination of Sennosides and Degraded Products in the Process of Sennoside Metabolism by HPLC
高效液相色谱法测定番泻甙及其代谢产物番泻素

Keywords: high performance liquid chromatography,sennosides A and B,sennidins A and,B
高效液相色谱
,番泻甙A和B,番泻素A和B

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Abstract:

A method for the separation and determination of sennosides A and B and the main composition (sennidins A and B) in degraded products of sennosides by linear gradient high performance liquid chromatography has been developed. Separation conditions were as follows: column, a Spherisorb C18 column (250 mm x 4.6 mm i.d., 10 microm); column temperature, 40 degrees C; detection wavelength, 360 nm; mobile phase A, 1.25% acetic acid aqueous solution; mobile phase B, methanol; linear gradient, 100% A --> (20 min) 100% B. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of real-time analysis in the process of sennoside metabolism.

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