%0 Journal Article
%T Determination of Sennosides and Degraded Products in the Process of Sennoside Metabolism by HPLC<br>高效液相色谱法测定番泻甙及其代谢产物番泻素
%A SUN Yan
%A LI Xuetuo
%A YU Xingju
%A <br>孙艳
%A 李雪驼
%A 虞星炬
%J 色谱
%D 2004
%I 
%X A method for the separation and determination of sennosides A and B and the main composition (sennidins A and B) in degraded products of sennosides by linear gradient high performance liquid chromatography has been developed. Separation conditions were as follows: column, a Spherisorb C18 column (250 mm x 4.6 mm i.d., 10 microm); column temperature, 40 degrees C; detection wavelength, 360 nm; mobile phase A, 1.25% acetic acid aqueous solution; mobile phase B, methanol; linear gradient, 100% A --> (20 min) 100% B. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of real-time analysis in the process of sennoside metabolism.
%K high performance liquid chromatography
%K sennosides A and B
%K sennidins A and
%K B<br>高效液相色谱
%K 番泻甙A和B
%K 番泻素A和B
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8762CACFE3B99CA8&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=B6DA1AC076E37400&eid=771152D1ADC1C0EB&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=7