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色谱  2004 

Isolation of β-Carotene from Discarded Tobacco Leaves
烟叶中β -胡萝卜素的分离纯化和测定

Keywords: NULL
烟叶
,β-胡萝卜素,分离,纯化,测定,结构鉴定,石油醚,直接柱色谱,废物利用

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Abstract:

Beta-carotene was isolated from discarded tobacco leaves by column chromatography using petroleum ether as eluent. Its structure was characterized by 1H-NMR and 13C-NMR spectra. The purity of the beta-carotene was determined to be 98% by a high performance liquid chromatographic (HPLC) method which was demonstrated to be accurate and reproducible. Infra-red spectrum and melting point of the isolated sample were also characterized in agreement with those reported in literature. The overall yield of 80%, the high purity and the easiness to recycle as the results of only a single solvent employed, would make the method an important reference for future research of beta-carotene and a favorable technique for future development.

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