%0 Journal Article
%T Isolation of β-Carotene from Discarded Tobacco Leaves<br>烟叶中β -胡萝卜素的分离纯化和测定
%A XIA Wei
%A CHEN Xinzhi
%A <br>夏薇
%A 陈新志
%J 色谱
%D 2004
%I 
%X Beta-carotene was isolated from discarded tobacco leaves by column chromatography using petroleum ether as eluent. Its structure was characterized by 1H-NMR and 13C-NMR spectra. The purity of the beta-carotene was determined to be 98% by a high performance liquid chromatographic (HPLC) method which was demonstrated to be accurate and reproducible. Infra-red spectrum and melting point of the isolated sample were also characterized in agreement with those reported in literature. The overall yield of 80%, the high purity and the easiness to recycle as the results of only a single solvent employed, would make the method an important reference for future research of beta-carotene and a favorable technique for future development.
%K NULL<br>烟叶
%K β-胡萝卜素
%K 分离
%K 纯化
%K 测定
%K 结构鉴定
%K 石油醚
%K 直接柱色谱
%K 废物利用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=12B0ADC8A215AEF4&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=318E4CC20AED4940&eid=014B591DF029732F&journal_id=1000-8713&journal_name=色谱&referenced_num=7&reference_num=10