Determination of alpha-acid in hop by high performance liquid chromatography]
高效液相色谱法测定酒花α-酸含量

A method for the determination of alpha-acid in hop by reversed-phase high performance liquid chromatgraphy is presented. The alpha-acid in hop and hop exteact was separated in a mu-Bondapak C18 cartridge by using V(CH3OH):V(H2O):V(H3PO4) = 80:20:0.25 as mobile phase. The flow rate was 1.0 mL/min and detection of alpha-acid was performed with UV detector at 314 nm. Quantification of alpha-acid was carried out by measuring peak height/peak area and comparing with external standard. The relative standard deviation of alpha-acid in hop was 2.02% and in hop extract was 1.78%. The method is simple, rapid and satisfactory.