%0 Journal Article
%T Determination of alpha-acid in hop by high performance liquid chromatography]<br>高效液相色谱法测定酒花α-酸含量
%A Chen Jiahua
%A Shen Libing
%A <br>陈家华
%A 沈礼兵
%J 色谱
%D 1999
%I 
%X A method for the determination of alpha-acid in hop by reversed-phase high performance liquid chromatgraphy is presented. The alpha-acid in hop and hop exteact was separated in a mu-Bondapak C18 cartridge by using V(CH3OH):V(H2O):V(H3PO4) = 80:20:0.25 as mobile phase. The flow rate was 1.0 mL/min and detection of alpha-acid was performed with UV detector at 314 nm. Quantification of alpha-acid was carried out by measuring peak height/peak area and comparing with external standard. The relative standard deviation of alpha-acid in hop was 2.02% and in hop extract was 1.78%. The method is simple, rapid and satisfactory.
%K NULL<br>高效液相色谱法
%K 啤酒花
%K 啤酒花抽提物
%K α-酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862B0AEC6D2EDDEFF1D&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=798FBE8DE1A255B1&eid=50BBDFAC8381694B&journal_id=1000-8713&journal_name=色谱&referenced_num=9&reference_num=2