Separation of Paroxetine and Its Intermediate Enantiomers by High Performance Liquid Chromatography Using Carboxy methyl-β-Cyclodextrin as Chiral Mobile Phase Additive
羧甲基-β-环糊精手性流动相添加剂法拆分帕罗西汀及其中间体对映体

A high performance liquid chromatographic method to separate the respective enantiomers of paroxetine and its intermediate was developed using chiral mobile phase additive. Separation was performed on a Diamond C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase was 0.1% phosphate acid-methanol (65 : 35, v/v) containing 0.38 g/L carboxymethyl-beta-cyclodextrin and the pH was adjusted to 7.2 by triethylamine. The detection wavelength was set at 210 nm and the temperature was 25 degrees C. With this method, paroxetine's trans/cis isomers and their enantiomers as well as intermediate HFP's trans/cis isomers and their enantiomers were separated simultaneously. The method is simple, rapid with high resolutions.