Preparative Separation of Aloin Diastereoisomers by High-Speed Countercurrent Chromatography Combined with Silica Gel Column Chromatography
高速逆流色谱与硅胶柱色谱结合分离制备高纯度芦荟素异构体

Aloin,naturally a mixture of two diastereoisomers,aloin A and aloin B,is the major(anthraquinone) in aloe,and now served as one of the important control constituents in most of the commercial aloe products.High-speed countercurrent chromatography(HSCCC) combined with(silica) gel column chromatography was developed for the preparative separation of the two individual(aloins).Aloin A(98%) and aloin B(96%) were obtained.Fast atom bombardment mass spectrometry(FAB-MS),()~1H nuclear magnetic resonance(()~1H NMR) and GOESY(gradient-enhanced nuclear Overhauser effect spectroscopy) were employed for the elucidation of their structure conformation.The developed method is of high preparative capacity and high efficiency in resolution.