%0 Journal Article
%T Preparative Separation of Aloin Diastereoisomers by High-Speed Countercurrent Chromatography Combined with Silica Gel Column Chromatography<br>高速逆流色谱与硅胶柱色谱结合分离制备高纯度芦荟素异构体
%A HUANG Danfeng
%A CAO Xueli
%A ZHAO Hua
%A DONG Yinmao
%A <br>黄丹凤
%A 曹学丽
%A 赵华
%A 董银卯
%J 色谱
%D 2006
%I 
%X Aloin,naturally a mixture of two diastereoisomers,aloin A and aloin B,is the major(anthraquinone) in aloe,and now served as one of the important control constituents in most of the commercial aloe products.High-speed countercurrent chromatography(HSCCC) combined with(silica) gel column chromatography was developed for the preparative separation of the two individual(aloins).Aloin A(98%) and aloin B(96%) were obtained.Fast atom bombardment mass spectrometry(FAB-MS),()~1H nuclear magnetic resonance(()~1H NMR) and GOESY(gradient-enhanced nuclear Overhauser effect spectroscopy) were employed for the elucidation of their structure conformation.The developed method is of high preparative capacity and high efficiency in resolution.
%K high-speed countercurrent chromatography(HSCCC)
%K silica gel column chromatography
%K preparative separation
%K high performance liquid chromatography(HPLC)
%K aloin diastereoisomers<br>高速逆流色谱
%K 硅胶柱色谱
%K 分离制备
%K 高效液相色谱
%K 芦荟素异构体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=99287F7C8D954FE4&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=ECE8E54D6034F642&eid=94E7F66E6C42FA23&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=18