Determination of the Adenosine Triphosphate in Myocardial Tissue by Ion-Pair Reversed-Phase High Performance Liquid Chromatography
反相离子对高效液相色谱法测定心肌组织中的三磷酸腺苷

ATP depletion is an important factor contributing to the incomplete recovery of ventricular function following ischemia and reperfusion. A simple and rapid method for determination of adenosine triphosphate(ATP) in myocardial tissue by RP-HPLC was developed. The chromatographic conditions were as follows: Spherisorb ODS2 column, V(40 mmol/L KH2PO4 and 5 mmol/L TBAP):V(methanol) = 74:26 (pH 6.2) mobile phase and UV detector at 259 nm. The ATP in myocardium was extracted with 0.4 mol/L HClO4. The calibration curve showed a good linearity in 5 mg/L-100 mg/L(r = 0.9998). The recovery ranged from 97.8% to 104.1%. The limit of detection was 2 mg/L. The verified results demonstrated that this method is precise, accurate and can be used for determination of ATP in myocardial tissue.