%0 Journal Article
%T Determination of the Adenosine Triphosphate in Myocardial Tissue by Ion-Pair Reversed-Phase High Performance Liquid Chromatography<br>反相离子对高效液相色谱法测定心肌组织中的三磷酸腺苷
%A ZHANG Yan-wan
%A LONG Cun
%A SHI Shi-yong
%A <br>张燕婉
%A 龙村
%A 史世勇
%J 色谱
%D 2000
%I 
%X ATP depletion is an important factor contributing to the incomplete recovery of ventricular function following ischemia and reperfusion. A simple and rapid method for determination of adenosine triphosphate(ATP) in myocardial tissue by RP-HPLC was developed. The chromatographic conditions were as follows: Spherisorb ODS2 column, V(40 mmol/L KH2PO4 and 5 mmol/L TBAP):V(methanol) = 74:26 (pH 6.2) mobile phase and UV detector at 259 nm. The ATP in myocardium was extracted with 0.4 mol/L HClO4. The calibration curve showed a good linearity in 5 mg/L-100 mg/L(r = 0.9998). The recovery ranged from 97.8% to 104.1%. The limit of detection was 2 mg/L. The verified results demonstrated that this method is precise, accurate and can be used for determination of ATP in myocardial tissue.
%K ion
%K pair reversed
%K phase high performance liquid chromatography
%K adenosine triphosphate
%K myocardial tissue<br>反相离子
%K 高效注相色谱法
%K 三磷酸腺苷
%K 心肌组织
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=9BA2AC08419A7FD8E7C1FB62BB7DB866&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=3224764AEAFCF8C2&eid=90612DF06FCE4D55&journal_id=1000-8713&journal_name=色谱&referenced_num=10&reference_num=3