Establishment and application of a two-dimensional liquid chromatographic system (NPLC×RPLC)
正相模式/反相模式的二维液相色谱系统的构建与应用

A two-dimensional liquid chromatographic system (NPLC x RPLC) has been developed. Normal phase liquid chromatography (NPLC) with a Hypersil SiO2 column (50 mm x 4.6 mm) was used as the first dimension, and reversed-phase liquid chromatography (RPLC) with a Kromasil C18 column (250 mm x 4.6 mm) was used as the second dimension. The interface was a ten-port, two-position valve with two storage loops. As to improve the compatibility of the two dimensional mobile phase and be good for adjusting the selectivity of separation, 1,4-dioxane was used in the mobile phase of the first dimension, and isopropyl alcohol was used in the mobile phase of the second dimension based on the characters of the organic solvents. By raising the temperature of the second dimension, the incompatibility of the two dimensional mobile phases was avoided. A Chinese potent medicine, Zhengtian Wan, was analyzed by this system. The total peak capacity reached 1 220.