%0 Journal Article
%T Establishment and application of a two-dimensional liquid chromatographic system (NPLC×RPLC)<br>正相模式/反相模式的二维液相色谱系统的构建与应用
%A LAN Tao
%A JIAO Fenglong
%A TANG Tao
%A WANG Fengyun
%A LI Tong
%A ZHANG Weibing
%A <br>兰韬
%A 焦丰龙
%A 唐涛
%A 王风云
%A 李彤
%A 张维冰
%J 色谱
%D 2008
%I 
%X A two-dimensional liquid chromatographic system (NPLC x RPLC) has been developed. Normal phase liquid chromatography (NPLC) with a Hypersil SiO2 column (50 mm x 4.6 mm) was used as the first dimension, and reversed-phase liquid chromatography (RPLC) with a Kromasil C18 column (250 mm x 4.6 mm) was used as the second dimension. The interface was a ten-port, two-position valve with two storage loops. As to improve the compatibility of the two dimensional mobile phase and be good for adjusting the selectivity of separation, 1,4-dioxane was used in the mobile phase of the first dimension, and isopropyl alcohol was used in the mobile phase of the second dimension based on the characters of the organic solvents. By raising the temperature of the second dimension, the incompatibility of the two dimensional mobile phases was avoided. A Chinese potent medicine, Zhengtian Wan, was analyzed by this system. The total peak capacity reached 1 220.
%K Two-dimensional LC
%K Sample loops-valve interface
%K Zhengtian Wan<br>二维液相色谱系统
%K 正相液相色谱/反相液相色谱联用
%K 定量环-阀切换接口
%K 正天丸
%K 中成药
%K 相模式
%K 液相
%K 色谱系统
%K 构建与应用
%K system
%K liquid
%K application
%K 峰容量
%K 分离分析
%K 样品
%K 正天丸
%K 中成药
%K 分离选择性
%K 调整
%K 兼容性
%K 色谱流动相
%K 改善
%K 异丙醇
%K 二氧六环
%K 特征
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=2C305B4F88ED602F3B9FC72A546CFEC6&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=5F8BAECF36EB55E2&eid=389106FB36CA8332&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=17