Determination of Water-Soluble Glucose, Fructose and Sucrose in Flue-Cured Tobacco by High Performance Anion Exchange Chromatography Coupled with Pulsed Amperometric Detection
高效阴离子交换色谱-脉冲安培检测法测定烤烟中的水溶性葡萄糖、果糖和蔗糖

The quantitative determination of water-soluble sugars such as glucose, fructose and sucrose in flue-cured tobacco by high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) was developed. The method was used to determine flue-cured tobacco samples containing large amounts of carbohydrates after pretreatment by water-leaching and membrane filtration. The separation was performed on a Dionex CarboPac PA-1 anion exchange column with 0.2 mol/L NaOH solution as the eluent at a flow rate of 1.0 mL/min. The injection volume was 20 microL. Good linearities were obtained for glucose, fructose and sucrose within the range of 0.5-100 mg/L (r2 > 0.997). The detection limits (S/N = 3) were 0.1 mg/L, 0.1 mg/L and 0.2 mg/L, and the average recoveries were 97.3%, 101.4% and 98.9% with good reproducibility for glucose, fructose and sucrose, respectively.