%0 Journal Article
%T Determination of Water-Soluble Glucose, Fructose and Sucrose in Flue-Cured Tobacco by High Performance Anion Exchange Chromatography Coupled with Pulsed Amperometric Detection<br>高效阴离子交换色谱-脉冲安培检测法测定烤烟中的水溶性葡萄糖、果糖和蔗糖
%A WANG Li
%A CHEN Qiaozhen
%A SONG Guoxin
%A SHEN Yi
%A LIU Baizhan
%A <br>王荔
%A 陈巧珍
%A 宋国新
%A 沈轶
%A 刘百战
%J 色谱
%D 2006
%I 
%X The quantitative determination of water-soluble sugars such as glucose, fructose and sucrose in flue-cured tobacco by high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) was developed. The method was used to determine flue-cured tobacco samples containing large amounts of carbohydrates after pretreatment by water-leaching and membrane filtration. The separation was performed on a Dionex CarboPac PA-1 anion exchange column with 0.2 mol/L NaOH solution as the eluent at a flow rate of 1.0 mL/min. The injection volume was 20 microL. Good linearities were obtained for glucose, fructose and sucrose within the range of 0.5-100 mg/L (r2 > 0.997). The detection limits (S/N = 3) were 0.1 mg/L, 0.1 mg/L and 0.2 mg/L, and the average recoveries were 97.3%, 101.4% and 98.9% with good reproducibility for glucose, fructose and sucrose, respectively.
%K high performance anion exchange chromatography(HPAEC)
%K pulsed amperometric detection(PAD)
%K glucose
%K fructose
%K sucrose
%K flue-cured tobacco<br>高效阴离子交换色谱
%K 脉冲安培检测法
%K 葡萄糖
%K 果糖
%K 蔗糖
%K 烤烟
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=5719295B1EC095F5&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=BC084ACE66B62CC8&eid=02DC3A182A5530DF&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=10