Separation and identification of bovine lactoferricin by high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry
高效液相色谱-基质辅助激光解吸电离串联飞行时间质谱分离鉴定牛乳铁蛋白素

A high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry(HPLC-MALDI-TOF/TOF MS) method was developed for the separation and identification of bovine lactoferricin(LfcinB).Bovine lactoferrin was hydrolyzed by pepsin and then separated by ion exchange chromatography and reversed-phase liquid chromatography(RP-LC).The antibacterial activities of the fractions from RP-LC separation were determined and the protein concentration of the fr...