%0 Journal Article
%T Separation and identification of bovine lactoferricin by high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry<br>高效液相色谱-基质辅助激光解吸电离串联飞行时间质谱分离鉴定牛乳铁蛋白素
%A AN Meichen
%A LIU Ning
%A <br>安美忱
%A 刘宁
%J 色谱
%D 2010
%I 
%X A high performance liquid chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry(HPLC-MALDI-TOF/TOF MS) method was developed for the separation and identification of bovine lactoferricin(LfcinB).Bovine lactoferrin was hydrolyzed by pepsin and then separated by ion exchange chromatography and reversed-phase liquid chromatography(RP-LC).The antibacterial activities of the fractions from RP-LC separation were determined and the protein concentration of the fr...
%K high performance liquid chromatography (HPLC)
%K matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS)
%K bovine lactoferricin
%K bovine lactoferrin<br>高效液相色谱
%K 基质辅助激光解吸电离串联飞行时间质谱
%K 牛乳铁蛋白素
%K 牛乳铁蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=04A50CA23CE7C010D10341B53018D797&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=F1A8654ADB4E656E&eid=798FBE8DE1A255B1&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=12