The Determination of Melatonin in Health Caring Medicine for Specific Purposes by High Performance Liquid Chromatography Mass Spectrometry
高效液相色谱-质谱法测定特定用途保健药品中的褪黑激素

A rapid and precise method for the determination of melatonin in health-caring medicine for specific purposes, based on high performance liquid chromatography-mass spectrometry, has been developed. The compound was identified using DAD UV spectrum and mass spectrum. Hypersil ODS column, 100 mm x 2.1 mm i.d., and mobile phase of methanol-water (70:30, V/V) were used for the assays. The quantitation was performed with external standard, and detection at 275 nm. The calibration curve of melatonin was linear in the range of 5-50 mg/L and the detection limit was ca. 0.2 ng.