Protein A Tangential Flow Membrane Chromatographic Column Used for Immunoadsorption Therapy
免疫吸附治疗用ProteinA切流膜色谱柱的研究

Tangential flow membrane chromatographic column is a new model of immunoadsorption therapy from blood. The results showed that the structure of the column and the flowing channel had a little damages for blood cells. The relation of flow-rate and backpressure with water, plasma and blood individually as the fluid flowing through the column has been studied. It was observed that backpressures increased with fluidviscosity, when flow-rate of blood was 120 mL/min and the backpressure reached 0. 093 MPa; The adsorption capacity of protein A of the column for IgG from human plasma has been measured. When plasma and blood individually circulated through the column for 1 h, the column with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23. 8 mg IgG/g dry matrix) from human plasma and 499. 4 mg IgG (21. 5 mg IgG/g dry matrix) from human blood respectively. The circulation time had a great influence on IgG adsorption capacity ; but the circulation rate had a little influence on IgG adsorption capacity. The result of extracoporeal blood per fusion for dog showed that the Protein A column has good blood biocompatibility.