%0 Journal Article
%T Protein A Tangential Flow Membrane Chromatographic Column Used for Immunoadsorption Therapy<br>免疫吸附治疗用ProteinA切流膜色谱柱的研究
%A Jia Lingyun
%A Yang Li
%A Zou Hanfa
%A Zhang Yukui
%A <br>贾凌云
%A 杨利
%A 邹汉法
%A 张玉奎
%A 赵久阳
%A 范成明
%A 沙丽艳
%J 色谱
%D 1999
%I 
%X Tangential flow membrane chromatographic column is a new model of immunoadsorption therapy from blood. The results showed that the structure of the column and the flowing channel had a little damages for blood cells. The relation of flow-rate and backpressure with water, plasma and blood individually as the fluid flowing through the column has been studied. It was observed that backpressures increased with fluidviscosity, when flow-rate of blood was 120 mL/min and the backpressure reached 0. 093 MPa; The adsorption capacity of protein A of the column for IgG from human plasma has been measured. When plasma and blood individually circulated through the column for 1 h, the column with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23. 8 mg IgG/g dry matrix) from human plasma and 499. 4 mg IgG (21. 5 mg IgG/g dry matrix) from human blood respectively. The circulation time had a great influence on IgG adsorption capacity ; but the circulation rate had a little influence on IgG adsorption capacity. The result of extracoporeal blood per fusion for dog showed that the Protein A column has good blood biocompatibility.
%K tangential flow
%K membrane chromatographic column
%K immunoadsorption
%K protein A
%K IgG<br>切流
%K 膜色谱柱
%K 免疫吸附
%K proteinA
%K IgG
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B86207C154CE36FEA443&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=D767283A3B658885&eid=480C51B1F0CE0AB6&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=3