%0 Journal Article %T Protein A Tangential Flow Membrane Chromatographic Column Used for Immunoadsorption Therapy
免疫吸附治疗用ProteinA切流膜色谱柱的研究 %A Jia Lingyun %A Yang Li %A Zou Hanfa %A Zhang Yukui %A
贾凌云 %A 杨利 %A 邹汉法 %A 张玉奎 %A 赵久阳 %A 范成明 %A 沙丽艳 %J 色谱 %D 1999 %I %X Tangential flow membrane chromatographic column is a new model of immunoadsorption therapy from blood. The results showed that the structure of the column and the flowing channel had a little damages for blood cells. The relation of flow-rate and backpressure with water, plasma and blood individually as the fluid flowing through the column has been studied. It was observed that backpressures increased with fluidviscosity, when flow-rate of blood was 120 mL/min and the backpressure reached 0. 093 MPa; The adsorption capacity of protein A of the column for IgG from human plasma has been measured. When plasma and blood individually circulated through the column for 1 h, the column with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23. 8 mg IgG/g dry matrix) from human plasma and 499. 4 mg IgG (21. 5 mg IgG/g dry matrix) from human blood respectively. The circulation time had a great influence on IgG adsorption capacity ; but the circulation rate had a little influence on IgG adsorption capacity. The result of extracoporeal blood per fusion for dog showed that the Protein A column has good blood biocompatibility. %K tangential flow %K membrane chromatographic column %K immunoadsorption %K protein A %K IgG
切流 %K 膜色谱柱 %K 免疫吸附 %K proteinA %K IgG %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B86207C154CE36FEA443&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=D767283A3B658885&eid=480C51B1F0CE0AB6&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=3