Gas chromatographic analysis of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir]
杉木针叶磷脂酰甘油脂肪酸组成分析

This paper reports a method for determination of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir with different cold-resistance. Some simplifications and improvements for analytical method of DEAE CL-6B column chromatography and of silica thin layer chromatography were also discussed. After being extracted with chloroform and methanol, phosphatidylglycerol was isolated from the general lipid extract by using DEAE CL-6B IEC and Silica TLC. Quantitative analysis was conducted by gas chromatography on a DB-FFAP (30m x 0.32mm i.d.) column. The results showed that there was a difference in the ratio of fatty acid (18:1+18:2+18:3)/16:0+16:1(3t)] between the two crosses of Chinese Fir with different cold-resistance, 1.12 for that with higher cold-resistance and 1.25 for that with lower cold-resistance. A special fatty acid 16:1(3t) was found in both of the two crosses.