%0 Journal Article
%T Gas chromatographic analysis of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir]<br>ɼľÕëÒ¶Á×Ö¬õ£¸ÊÓÍÖ¬·¾Ëá×é³É·ÖÎö
%A H Bao
%A M Li
%A Z Hu
%A Z He
%A J Shi
%A <br>°üºê
%A Àî÷
%A ºú×ÈÜß
%A ºÎìõÏé
%A Ê©¼¾É­
%J É«Æ×
%D 1997
%I 
%X This paper reports a method for determination of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir with different cold-resistance. Some simplifications and improvements for analytical method of DEAE CL-6B column chromatography and of silica thin layer chromatography were also discussed. After being extracted with chloroform and methanol, phosphatidylglycerol was isolated from the general lipid extract by using DEAE CL-6B IEC and Silica TLC. Quantitative analysis was conducted by gas chromatography on a DB-FFAP (30m x 0.32mm i.d.) column. The results showed that there was a difference in the ratio of fatty acid (18:1+18:2+18:3)/16:0+16:1(3t)] between the two crosses of Chinese Fir with different cold-resistance, 1.12 for that with higher cold-resistance and 1.25 for that with lower cold-resistance. A special fatty acid 16:1(3t) was found in both of the two crosses.
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%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D6AB3E36DD530DB78&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=0B39A22176CE99FB&sid=F122871CC7EC92DC&eid=6FBD78E3BAB60869&journal_id=1000-8713&journal_name=É«Æ×&referenced_num=6&reference_num=3