Studies on the Methods of Increasing Solute Concentration in Determination of Adsorption Isotherms of Proteins with Frontal Chromatography
用前沿色谱法测定蛋白质吸附等温线时增浓方式的研究

dsorption isotherms of proteins on the surface of the hydrophobic sorbents were measured by consecu-tive and non-consecutive frontal chromatography.It was found that significant difference exsits between theabove two methods. The difference is attributed to the unreliabilitv in the determination of the slope of theinflexion point curve in frontal chromatography,the kenetic factors in chromatographic process and the prob-lems that intrinsically exsit in the two methods. A suggestion which is possible to solve this problem is alsoraised.