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色谱  1995 

Studies on the Coupling Technique between High Performance Capillary Electrophoresis and Charge-Coupled Device Detector I.A Set-up for Multiwavelength Fluorescence Detection
高效毛细管电泳-电荷耦合器件检测器联用技术研究Ⅰ.多波长荧光检测装置

Keywords: high performance capillary electrophoresis,charge-coupled device detector,fluorescence detection,fluorescein,rhodamine B
高效毛细管电泳,电荷耦合器件检测器,荧光检测,荧光素,罗丹明B

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Abstract:

new instrumental set-up combining capillary electrophoresis separation and charge-coupled device mul-tiwavelength fluorescence detection is described.The performance of the set-up was evaluated with fluores-cein and rhodamine B. A three-dimensional electrophorogram that showed the spectra and time resolutionwas obtained,on which the separated species could be identified and determined in comparing with their stan-dard fluorescence spectra.

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